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anti his6 hrp ab  (SouthernBiotech)


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    Structured Review

    SouthernBiotech anti his6 hrp ab
    (a) Nanobodies targeting the SARS-CoV-2 spike protein and murine TNF, as well as the TNF itself, were produced on MANGO from CFPS reactions (b) After production, the products, including TNF (predominantly monomer with traces of dimer), TNF Nb, and anti-spike Nb (Nb21), were analyzed using 4-20% gradient SDS-PAGE and stained with ProBlue Safe, which confirmed their presence at the correct size. The molecular weight ladder (in kilodaltons) is shown on the left. (c) ELISA was performed against recombinant spike protein receptor-binding domain (RBD) using MANGO-produced anti-spike Nb21 as the primary antibody and <t>HRP-conjugated</t> anti-His₆ antibody as the secondary. Detection was performed using the QuantaBlu fluorogenic peroxidase substrate kit, and fluorescence intensity was measured in a conventional plate reader. (d) TNF neutralization assay was conducted using anti-TNF Nb (MANGO) and L929 cells, demonstrating (e) cell survival after preincubation of TNF (1 ng/mL) with both in-house and commercial antibodies. (f) Dose-response curves in the presence or absence of MANGO anti-TNF Nb. Luminescence was measured with a standard plate reader. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. Data are shown as the mean of three technical replicates ± SD (ns p-value > 0.05, **** p-value < 0.0001). Abbreviations: TNF, tumor necrosis factor; TNFR, tumor necrosis factor receptor; Nb, nanobody.
    Anti His6 Hrp Ab, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+his6+hrp+ab/Mouse+Anti-His-Tag-HRP/med_rxiv__2025__10__15__25338083-340-16-18
    Average 94 stars, based on 21 article reviews
    anti his6 hrp ab - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Automated Cell-free Protein Synthesis for Distributed Biomanufacturing"

    Article Title: Automated Cell-free Protein Synthesis for Distributed Biomanufacturing

    Journal: medRxiv

    doi: 10.1101/2025.10.15.25338083

    (a) Nanobodies targeting the SARS-CoV-2 spike protein and murine TNF, as well as the TNF itself, were produced on MANGO from CFPS reactions (b) After production, the products, including TNF (predominantly monomer with traces of dimer), TNF Nb, and anti-spike Nb (Nb21), were analyzed using 4-20% gradient SDS-PAGE and stained with ProBlue Safe, which confirmed their presence at the correct size. The molecular weight ladder (in kilodaltons) is shown on the left. (c) ELISA was performed against recombinant spike protein receptor-binding domain (RBD) using MANGO-produced anti-spike Nb21 as the primary antibody and HRP-conjugated anti-His₆ antibody as the secondary. Detection was performed using the QuantaBlu fluorogenic peroxidase substrate kit, and fluorescence intensity was measured in a conventional plate reader. (d) TNF neutralization assay was conducted using anti-TNF Nb (MANGO) and L929 cells, demonstrating (e) cell survival after preincubation of TNF (1 ng/mL) with both in-house and commercial antibodies. (f) Dose-response curves in the presence or absence of MANGO anti-TNF Nb. Luminescence was measured with a standard plate reader. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. Data are shown as the mean of three technical replicates ± SD (ns p-value > 0.05, **** p-value < 0.0001). Abbreviations: TNF, tumor necrosis factor; TNFR, tumor necrosis factor receptor; Nb, nanobody.
    Figure Legend Snippet: (a) Nanobodies targeting the SARS-CoV-2 spike protein and murine TNF, as well as the TNF itself, were produced on MANGO from CFPS reactions (b) After production, the products, including TNF (predominantly monomer with traces of dimer), TNF Nb, and anti-spike Nb (Nb21), were analyzed using 4-20% gradient SDS-PAGE and stained with ProBlue Safe, which confirmed their presence at the correct size. The molecular weight ladder (in kilodaltons) is shown on the left. (c) ELISA was performed against recombinant spike protein receptor-binding domain (RBD) using MANGO-produced anti-spike Nb21 as the primary antibody and HRP-conjugated anti-His₆ antibody as the secondary. Detection was performed using the QuantaBlu fluorogenic peroxidase substrate kit, and fluorescence intensity was measured in a conventional plate reader. (d) TNF neutralization assay was conducted using anti-TNF Nb (MANGO) and L929 cells, demonstrating (e) cell survival after preincubation of TNF (1 ng/mL) with both in-house and commercial antibodies. (f) Dose-response curves in the presence or absence of MANGO anti-TNF Nb. Luminescence was measured with a standard plate reader. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. Data are shown as the mean of three technical replicates ± SD (ns p-value > 0.05, **** p-value < 0.0001). Abbreviations: TNF, tumor necrosis factor; TNFR, tumor necrosis factor receptor; Nb, nanobody.

    Techniques Used: Produced, SDS Page, Staining, Molecular Weight, Enzyme-linked Immunosorbent Assay, Recombinant, Binding Assay, Fluorescence, Neutralization

    Related Articles

    Incubation:

    Article Title: Automated Cell-free Protein Synthesis for Distributed Biomanufacturing
    Article Snippet: Throughout all procedures, endotoxin-free ultrapure water (Sigma-Aldrich, TMS-011-A) was used. .. Membranes were blocked for 1 hour with 5% skim milk (Bioshop) in TBST, then incubated for 1 hour with anti-His6-HRP Ab (SouthernBiotech, 4603-05, 1:8,000 dilution) in 0.05% milk in TBST. .. After five TBST washes (each lasting 5 minutes), the membranes were developed using Clarity Max ECL substrate (Bio-Rad) and imaged with the colorimetric and chemiluminescent settings of the Bio-Rad ChemiDoc MP Imaging System.

    Article Title: Automated Cell-free Protein Synthesis for Distributed Biomanufacturing
    Article Snippet: After five TBST washes (each lasting 5 minutes), the membranes were developed using Clarity Max ECL substrate (Bio-Rad) and imaged with the colorimetric and chemiluminescent settings of the Bio-Rad ChemiDoc MP Imaging System. .. After six additional washes with PBST, wells were incubated for 1 hour at 350 rpm with anti-His6-HRP Ab (SouthernBiotech, 4603-05, 1:6,000) prepared in 0.5% skim milk (Bioshop) in PBST (Gibco). .. Black 384-well plates were coated overnight at 4 °C with RBD antigen (SinoBiological [40592-VNAH], 1 μg/mL in PBS).



    Similar Products

    anti his6 hrp ab  (SouthernBiotech)
    94
    SouthernBiotech anti his6 hrp ab
    (a) Nanobodies targeting the SARS-CoV-2 spike protein and murine TNF, as well as the TNF itself, were produced on MANGO from CFPS reactions (b) After production, the products, including TNF (predominantly monomer with traces of dimer), TNF Nb, and anti-spike Nb (Nb21), were analyzed using 4-20% gradient SDS-PAGE and stained with ProBlue Safe, which confirmed their presence at the correct size. The molecular weight ladder (in kilodaltons) is shown on the left. (c) ELISA was performed against recombinant spike protein receptor-binding domain (RBD) using MANGO-produced anti-spike Nb21 as the primary antibody and <t>HRP-conjugated</t> anti-His₆ antibody as the secondary. Detection was performed using the QuantaBlu fluorogenic peroxidase substrate kit, and fluorescence intensity was measured in a conventional plate reader. (d) TNF neutralization assay was conducted using anti-TNF Nb (MANGO) and L929 cells, demonstrating (e) cell survival after preincubation of TNF (1 ng/mL) with both in-house and commercial antibodies. (f) Dose-response curves in the presence or absence of MANGO anti-TNF Nb. Luminescence was measured with a standard plate reader. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. Data are shown as the mean of three technical replicates ± SD (ns p-value > 0.05, **** p-value < 0.0001). Abbreviations: TNF, tumor necrosis factor; TNFR, tumor necrosis factor receptor; Nb, nanobody.
    Anti His6 Hrp Ab, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+his6+hrp+ab/Mouse+Anti-His-Tag-HRP/med_rxiv__2025__10__15__25338083-340-16-18
    Average 94 stars, based on 1 article reviews
    anti his6 hrp ab - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) Nanobodies targeting the SARS-CoV-2 spike protein and murine TNF, as well as the TNF itself, were produced on MANGO from CFPS reactions (b) After production, the products, including TNF (predominantly monomer with traces of dimer), TNF Nb, and anti-spike Nb (Nb21), were analyzed using 4-20% gradient SDS-PAGE and stained with ProBlue Safe, which confirmed their presence at the correct size. The molecular weight ladder (in kilodaltons) is shown on the left. (c) ELISA was performed against recombinant spike protein receptor-binding domain (RBD) using MANGO-produced anti-spike Nb21 as the primary antibody and HRP-conjugated anti-His₆ antibody as the secondary. Detection was performed using the QuantaBlu fluorogenic peroxidase substrate kit, and fluorescence intensity was measured in a conventional plate reader. (d) TNF neutralization assay was conducted using anti-TNF Nb (MANGO) and L929 cells, demonstrating (e) cell survival after preincubation of TNF (1 ng/mL) with both in-house and commercial antibodies. (f) Dose-response curves in the presence or absence of MANGO anti-TNF Nb. Luminescence was measured with a standard plate reader. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. Data are shown as the mean of three technical replicates ± SD (ns p-value > 0.05, **** p-value < 0.0001). Abbreviations: TNF, tumor necrosis factor; TNFR, tumor necrosis factor receptor; Nb, nanobody.

    Journal: medRxiv

    Article Title: Automated Cell-free Protein Synthesis for Distributed Biomanufacturing

    doi: 10.1101/2025.10.15.25338083

    Figure Lengend Snippet: (a) Nanobodies targeting the SARS-CoV-2 spike protein and murine TNF, as well as the TNF itself, were produced on MANGO from CFPS reactions (b) After production, the products, including TNF (predominantly monomer with traces of dimer), TNF Nb, and anti-spike Nb (Nb21), were analyzed using 4-20% gradient SDS-PAGE and stained with ProBlue Safe, which confirmed their presence at the correct size. The molecular weight ladder (in kilodaltons) is shown on the left. (c) ELISA was performed against recombinant spike protein receptor-binding domain (RBD) using MANGO-produced anti-spike Nb21 as the primary antibody and HRP-conjugated anti-His₆ antibody as the secondary. Detection was performed using the QuantaBlu fluorogenic peroxidase substrate kit, and fluorescence intensity was measured in a conventional plate reader. (d) TNF neutralization assay was conducted using anti-TNF Nb (MANGO) and L929 cells, demonstrating (e) cell survival after preincubation of TNF (1 ng/mL) with both in-house and commercial antibodies. (f) Dose-response curves in the presence or absence of MANGO anti-TNF Nb. Luminescence was measured with a standard plate reader. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. Data are shown as the mean of three technical replicates ± SD (ns p-value > 0.05, **** p-value < 0.0001). Abbreviations: TNF, tumor necrosis factor; TNFR, tumor necrosis factor receptor; Nb, nanobody.

    Article Snippet: After six additional washes with PBST, wells were incubated for 1 hour at 350 rpm with anti-His6-HRP Ab (SouthernBiotech, 4603-05, 1:6,000) prepared in 0.5% skim milk (Bioshop) in PBST (Gibco).

    Techniques: Produced, SDS Page, Staining, Molecular Weight, Enzyme-linked Immunosorbent Assay, Recombinant, Binding Assay, Fluorescence, Neutralization